Osteopontin Rekombinanter Antikörper

Osteopontin Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

6B11

Kat-Nr. : 80912-4-PBS

Synonyme

OPN, SPP1, SPP1, 6B11, BNSP, Bone sialoprotein 1

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  WB analysis using 80912-4-RR (same clone as 80912-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80912-4-RR (OPN, SPP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80912-4-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 80912-4-RR (same clone as 80912-4-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug OPN, SPP1 Recombinant antibody (80912-4-RR, Clone:6B11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80912-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80912-4-RR

  Biolayer interferometry (BLl) kinetic assays of 80912-4-RR against Human OPN, SPP1 were performed. The affinity constant is 199 nM.

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Geprüfte Anwendungen

Produktinformation

80912-4-PBS bindet in WB, FC (Intra), Indirect ELISA Osteopontin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Osteopontin fusion protein Ag19216
• Vollständiger Name: secreted phosphoprotein 1
• Berechnetes Molekulargewicht: 314 aa, 35 kDa
• Beobachtetes Molekulargewicht: 70 kDa, 44-66 kDa
• GenBank-Zugangsnummer: BC007016
• Gene symbol: Osteopontin
• Gene ID (NCBI): 6696
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands corresponding to peptide fragments (PMID: 8195113; 17890765).