OPN1SW Polyklonaler Antikörper

OPN1SW Polyklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 24660-1-AP

Synonyme

Blue sensitive opsin, Blue-sensitive opsin, BOP, CBT, Short-wave-sensitive opsin 1

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  IHC staining of mouse eye using 24660-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24660-1-AP (OPN1SW antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 24660-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using OPN1SW antibody (24660-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse eye using 24660-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using OPN1SW antibody (24660-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat eye using 24660-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using OPN1SW antibody (24660-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maus-Augengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Augengewebe, Ratten-Augengewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24660-1-AP bindet in IHC, IF-P, ELISA OPN1SW und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OPN1SW fusion protein Ag20387
• Vollständiger Name: opsin 1 (cone pigments), short-wave-sensitive
• Berechnetes Molekulargewicht: 348 aa, 39 kDa
• GenBank-Zugangsnummer: BC156719
• Gene symbol: OPN1SW
• Gene ID (NCBI): 611
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OPN1SW (Short-wave-sensitive opsin 1) belongs to the G-protein coupled receptor 1 family, opsin subfamily. It's one of three types of cone photoreceptors responsible for normal color vision. Inherited tritan color vision deficiencies exhibit an autosomal dominant inheritance pattern and are caused by mutations in OPN1SW (PMID: 32400513).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christoffer (Verified Customer) (02-19-2025)

The antibody performs well on both cryosections and paraffin-embedded tissues. However, to ensure optimal results, antigen retrieval is advised for cryosections. The citrate buffer antigen retrieval technique was utilized

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: Adult mouse retina / cone photoreceptor cells