OPRL1 Polyklonaler Antikörper

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• KD/KO Validated

OPRL1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12970-1-PBS

Synonyme

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  WB analysis of HeLa using 12970-1-AP (same clone as 12970-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12970-1-AP (OPRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12970-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 12970-1-AP (same clone as 12970-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12970-1-AP (OPRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12970-1-PBS in a different storage buffer formulation.

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  IP experiment of SH-SY5Y using 12970-1-AP (same clone as 12970-1-PBS)

  IP result of anti-OPRL1 (IP:12970-1-AP, 4ug; Detection:12970-1-AP 1:500) with SH-SY5Y cells lysate 2000ug. This data was developed using the same antibody clone with 12970-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12970-1-AP (same clone as 12970-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12970-1-AP (OPRL1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12970-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12970-1-AP (same clone as 12970-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12970-1-AP (OPRL1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 12970-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12970-1-AP (same clone as 12970-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12970-1-AP (OPRL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12970-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12970-1-PBS bindet in WB, IHC, IP, Indirect ELISA OPRL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OPRL1 fusion protein Ag4079
• Vollständiger Name: opiate receptor-like 1
• Berechnetes Molekulargewicht: 370 aa, 41 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC038433
• Gene symbol: OPRL1
• Gene ID (NCBI): 4987
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The OPRL1 (opioid related nociceptin receptor 1) is the G-protein coupled receptor activated by the endogenou NC (neuropeptide nociceptin) (PMID:7566152). OPRL1 is widely expressed in the central nervous system, including the hippocampal dentate gyrus, cortical areas, striatum, thalamus, and hypothalamus (PMID:19501074). OPRL1 has been found to play an essential role in cognition, and to modulate inflammation and immune responses (PMID:30152845).