OPTN/Optineurin Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

OPTN/Optineurin Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

6C1H4

Kat-Nr. : 60293-1-Ig

Synonyme

Optineurin, OPTN, 6C1H4, ALS12, E3-14.7K-interacting protein

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  WB analysis using 60293-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60293-1-Ig (OPTN/Optineurin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60293-1-Ig

  WB result of OPTN/Optineurin antibody (60293-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OPTN/Optineurin transfected HeLa cells.

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  IHC staining of human pancreas cancer using 60293-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60293-1-Ig (OPTN Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas cancer using 60293-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60293-1-Ig (OPTN Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 60293-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60293-1-Ig (OPTN Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 60293-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60293-1-Ig (OPTN Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MCF-7 using 60293-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using OPTN antibody (60293-1-Ig, Clone: 6C1H4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, Hausschwein-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

60293-1-Ig bindet in WB, IHC, IF/ICC, ELISA OPTN/Optineurin und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: OPTN/Optineurin fusion protein Ag1272
• Vollständiger Name: optineurin
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 70-74 kDa
• GenBank-Zugangsnummer: BC013876
• Gene symbol: OPTN
• Gene ID (NCBI): 10133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork.

Publikationen

Species	Application	Title
human	WB,IF	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv ; KD Validated
human	WB	Autophagy; Copper-dependent autophagic degradation of GPX4 drives ferroptosis; Authors - Qian Xue