OPTN/Optineurin Monoklonaler Antikörper

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• KD/KO Validated

OPTN/Optineurin Monoklonal Antikörper für WB, IHC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC

Konjugation

Biotin

CloneNo.

6C1H4

Kat-Nr. : Biotin-60293

Synonyme

Optineurin, OPTN, FIP2, FIP 2, FAA4

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  WB analysis of human brain using Biotin-60293

  Fetal human brain was subjected to SDS-PAGE followed by western blot with Biotin-60293 (biotin labeled OPTN monoclonal antibody) at dilution of 1:1000. Avidin-HRP was used instead of secondary antibody.

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  IHC staining of human pancreas cancer using Biotin-60293

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using Biotin-60293 (OPTN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using Biotin-60293

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using Biotin-60293 (OPTN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human brain
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-60293 bindet in WB, IHC OPTN/Optineurin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: OPTN/Optineurin fusion protein Ag1272
• Vollständiger Name: optineurin
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 70-74 kDa
• GenBank-Zugangsnummer: BC013876
• Gene symbol: OPTN
• Gene ID (NCBI): 10133
• Konjugation: Biotin
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. This antibody is conjugated with Biotin.