ORAI3 Polyklonaler Antikörper

ORAI3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Rezensionen (1)

Kat-Nr. : 25766-1-AP

Synonyme

Protein orai-3, TMEM142C, Transmembrane protein 142C

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  WB analysis using 25766-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25766-1-AP (ORAI3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 25766-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25766-1-AP (ORAI3 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human lung cancer using 25766-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25766-1-AP (ORAI3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, BxPC-3-Zellen, LNCaP-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

25766-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA ORAI3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ORAI3 fusion protein Ag22513
• Vollständiger Name: ORAI calcium release-activated calcium modulator 3
• Berechnetes Molekulargewicht: 295 aa, 32 kDa
• Beobachtetes Molekulargewicht: ~30 kDa, 60 kDa
• GenBank-Zugangsnummer: BC006126
• Gene symbol: ORAI3
• Gene ID (NCBI): 93129
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ORAI3, also named TMEM142C, is a key regulator or component of store-operated Ca2+ channels and transcription factor NFAT nuclear import. ORAI3 channels appeared to differ from Orai1 and -2 in being somewhat resistant to Ca2+ depotentiation. The molecular weight of monomers and dimers for Orai3 is about 30-35 kDa and 60-70 kDa (PMID: 20971921; 17293345; 22580508).

Publikationen

Species	Application	Title
human	WB	Sci Signal; ALOX5 drives the pyroptosis of CD4+ T cells and tissue inflammation in rheumatoid arthritis; Authors - Hao Cai
human	WB,IF,CoIP	BMJ Open Diabetes Res Care; Orai-IGFBP3 signaling complex regulates high-glucose exposure-induced increased proliferation, permeability, and migration of human coronary artery endothelial cells.; Authors - Suwen Bai
mouse	IP	BMJ Open Diabetes Res Care; Orai-vascular endothelial-cadherin signaling complex regulates high-glucose exposure-induced increased permeability of mouse aortic endothelial cells.; Authors - Yuan Wei
human	WB	Exp Ther Med; Resveratrol sensitizes A549 cells to irradiation damage via suppression of store-operated calcium entry with Orai1 and STIM1 downregulation.; Authors - Wu Lele
rat	WB	Allergy Asthma Immunol Res; Exercise Reduces Airway Smooth Muscle Contraction in Asthmatic Rats via Inhibition of IL-4 Secretion and Store-Operated Ca2+ Entry Pathway; Authors - Jun-Hao Huang
mouse	IF	J Orthop Translat; Tibial cortex transverse transport regulates Orai1/STIM1-mediated NO release and improve the migration and proliferation of vessels via increasing osteopontin expression; Authors - Lingchao Kong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

David (Verified Customer) (02-06-2023)

Works pretty well for our WB

• Applications: Western Blot