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ORAI3 Rekombinanter Antikörper

ORAI3 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241568A2

Kat-Nr. : 84340-2-PBS

Synonyme

Transmembrane protein 142C, TMEM142C, Protein orai-3, 241568A2

Filter:
  • Western Blot (WB) analysis of various lysates using ORAI3 Recombinant antibody (84340-2-RR)

    WB analysis using 84340-2-RR (same clone as 84340-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84340-2-RR (ORAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84340-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using ORAI3 Recombinant antibody (84340-2-RR)

    WB analysis of MCF-7 using 84340-2-RR (same clone as 84340-2-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 84340-2-RR (ORAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84340-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using ORAI3 Recombinant antibody (84340-2-RR)

    IF Staining of Jurkat using 84340-2-RR (same clone as 84340-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ORAI3 antibody (84340-2-RR, Clone: 241568A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84340-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01228-1

    Cytometric bead array standard curve of MP01228-1

    Cytometric bead array standard curve of MP01228-1, ORAI3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84340-3-PBS. Detection antibody: 84340-2-PBS. Standard: Ag36135. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84340-2-RR

    Affinity and Kinetic Characterization of 84340-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84340-2-RR against Human ORAI3 were performed. The affinity constant is 1.24 nM.

Geprüfte Anwendungen

Produktinformation

84340-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA ORAI3 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen ORAI3 fusion protein Ag36135
Vollständiger Name ORAI calcium release-activated calcium modulator 3
Berechnetes Molekulargewicht 295 aa, 32 kDa
Beobachtetes Molekulargewicht ~30 kDa，~60 kDa
GenBank-ZugangsnummerBC006126
Gene symbol ORAI3
Gene ID (NCBI) 93129
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ORAI3, also named TMEM142C, is a key regulator or component of store-operated Ca2+ channels and transcription factor NFAT nuclear import. ORAI3 channels appeared to differ from Orai1 and -2 in being somewhat resistant to Ca2+ depotentiation. The molecular weight of monomers and dimers for Orai3 is about 30-35 kDa and 60-70 kDa (PMID: 20971921; 17293345; 22580508).