ORAI3 Rekombinanter Antikörper

ORAI3 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241568A2

Kat-Nr. : 84340-2-RR

Synonyme

Transmembrane protein 142C, TMEM142C, Protein orai-3, 241568A2

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  WB analysis using 84340-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84340-2-RR (ORAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 84340-2-RR

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 84340-2-RR (ORAI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 84340-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ORAI3 antibody (84340-2-RR, Clone: 241568A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84340-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84340-2-RR against Human ORAI3 were performed. The affinity constant is 1.24 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A549-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84340-2-RR bindet in WB, IF/ICC, ELISA ORAI3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ORAI3 fusion protein Ag36135
• Vollständiger Name: ORAI calcium release-activated calcium modulator 3
• Berechnetes Molekulargewicht: 295 aa, 32 kDa
• Beobachtetes Molekulargewicht: ~30 kDa，~60 kDa
• GenBank-Zugangsnummer: BC006126
• Gene symbol: ORAI3
• Gene ID (NCBI): 93129
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ORAI3, also named TMEM142C, is a key regulator or component of store-operated Ca2+ channels and transcription factor NFAT nuclear import. ORAI3 channels appeared to differ from Orai1 and -2 in being somewhat resistant to Ca2+ depotentiation. The molecular weight of monomers and dimers for Orai3 is about 30-35 kDa and 60-70 kDa (PMID: 20971921; 17293345; 22580508).