ORM1/2 Polyklonaler Antikörper

ORM1/2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11199-1-PBS

Synonyme

ORM2, AGP 2, AGP B, AGP B', AGP2

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  WB analysis using 11199-1-AP (same clone as 11199-1-PBS)

  human plasma lysates were subjected to SDS PAGE followed by western blot with 11199-1-AP (ORM1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

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  WB analysis using 11199-1-AP (same clone as 11199-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11199-1-AP (ORM1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

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  IP experiment of L02 using 11199-1-AP (same clone as 11199-1-PBS)

  IP result of anti-ORM1/2 (IP:11199-1-AP, 3ug; Detection:11199-1-AP 1:800) with L02 cells lysate 2500ug. This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11199-1-AP (same clone as 11199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11199-1-AP (ORM1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11199-1-AP (same clone as 11199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11199-1-AP (ORM1/2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 11199-1-AP (same clone as 11199-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ORM1/2 antibody (11199-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11199-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11199-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA ORM1/2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ORM1/2 fusion protein Ag1667
• Vollständiger Name: orosomucoid 2
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 24-28 kDa, 41-47 kDa
• GenBank-Zugangsnummer: BC015964
• Gene symbol: ORM2
• Gene ID (NCBI): 5005
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ORM2, also named as AGP2 and OMD 2, belongs to the calycin superfamily and Lipocalin family. It functions as transport protein in the blood stream. ORM2 binds various hydrophobic ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability. ORM2 appears to function in modulating the activity of the immune system during the acute-phase reaction. ORM2 is one of the conserved endoplasmic reticulum membrane proteins which regulating lipid homeostasis and protein quality control. This antibody can recognize both ORM1 and ORM2.