ORM1/2 Polyklonaler Antikörper

ORM1/2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 11199-1-AP

Synonyme

AGP 2, AGP B, AGP B', AGP2, Alpha 1 acid glycoprotein 2, OMD 2, ORM1/2, ORM2, orosomucoid 2

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Galerie der Validierungsdaten

• 

  WB analysis using 11199-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11199-1-AP (ORM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11199-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11199-1-AP (ORM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11199-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11199-1-AP (ORM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 11199-1-AP

  IP Result of anti-ORM2 (IP:11199-1-AP, 3ug; Detection:11199-1-AP 1:800) with L02 cells lysate 2500ug.

• 

  IHC staining of human liver using 11199-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11199-1-AP (ORM1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11199-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11199-1-AP (ORM1/2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11199-1-AP

  Immunofluorescent analysis of Hela cells, using ORM2 antibody 11199-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, A549-Zellen, Mauslebergewebe
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

11199-1-AP bindet in WB, IP, IHC, IF, ELISA ORM1/2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ORM1/2 fusion protein Ag1667
• Vollständiger Name: orosomucoid 2
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 24-28 kDa, 41-47 kDa
• GenBank-Zugangsnummer: BC015964
• Gene symbol: ORM2
• Gene ID (NCBI): 5005
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ORM2, also named as AGP2 and OMD 2, belongs to the calycin superfamily and Lipocalin family. It functions as transport protein in the blood stream. ORM2 binds various hydrophobic ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability. ORM2 appears to function in modulating the activity of the immune system during the acute-phase reaction. ORM2 is one of the conserved endoplasmic reticulum membrane proteins which regulating lipid homeostasis and protein quality control. This antibody can recognize both ORM1 and ORM2.

Publikationen

Species	Application	Title
mouse		Carcinogenesis; FXR-Gankyrin axis is involved in development of pediatric liver cancer.; Authors - Leila Valanejad
mouse	WB	Mol Cell Biol; RNA Binding Protein CUGBP1 Inhibits Liver Cancer in a Phosphorylation-Dependent Manner.; Authors - Kyle Lewis
human	WB	BMC Cancer; Differential profiling of breast cancer plasma proteome by isotope-coded affinity tagging method reveals biotinidase as a breast cancer biomarker.; Authors - Kang Un-Beom UB

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hanyang (Verified Customer) (03-03-2022)

Considering results we got, the capacity of antibody is regarded as suspicious.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:400

ORM1/2 Antibody Immunohistochemistry,Immunofluorescence validation (1:400 dilution) in (Cat no:11199-1-AP)

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