ORM2-Specific Monoklonaler Antikörper

ORM2-Specific Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

4E9H10

Kat-Nr. : 66217-1-Ig

Synonyme

ORM2, 4E9H10, AGP 2, AGP B, AGP B'

---

• 

  WB analysis of human plasma using 66217-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66217-1-Ig (ORM2-Specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 66217-1-Ig

  Recombinant protein of ORM1 and ORM2 were subjected to SDS PAGE followed by western blot with 66217-1-Ig (ORM2-Specific antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66217-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66217-1-Ig (ORM2-Specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66217-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66217-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66217-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66217-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66217-1-Ig (ORM2-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66217-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ORM2-Specific antibody (66217-1-Ig, Clone: 4E9H10 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe, A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66217-1-Ig bindet in WB, IHC, IF/ICC, ELISA ORM2-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ORM2-Specific fusion protein Ag18984
• Vollständiger Name: orosomucoid 2
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC015964
• Gene symbol: ORM2
• Gene ID (NCBI): 5005
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ORM2, also named as AGP2 and OMD 2, belongs to the calycin superfamily and Lipocalin family. It functions as transport protein in the blood stream. ORM2 binds various hydrophobic ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability. ORM2 appears to function in modulating the activity of the immune system during the acute-phase reaction. ORM2 is one of the conserved endoplasmic reticulum membrane proteins which regulating lipid homeostasis and protein quality control. This antibody can specially recognize ORM2. It recognizes a band about 45 kDa in human plasma which may be due to the glycosylation of ORM2 or the dimer formation of the protein.