OS9 Polyklonaler Antikörper

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• KD/KO Validated

OS9 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 10061-1-AP

Synonyme

Amplified in osteosarcoma 9, Protein OS-9

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  WB analysis of HEK-293 using 10061-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10061-1-AP (OS9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10061-1-AP

  WB result of OS9 antibody (10061-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-OS9 transfected MCF-7 cells.

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  WB analysis of HeLa using 10061-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10061-1-AP (OS9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10061-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10061-1-AP (OS9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10061-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10061-1-AP (OS9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10061-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10061-1-AP (OS9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10061-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10061-1-AP (OS9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10061-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using OS9 antibody (10061-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IF: See 1 publications below

Produktinformation

10061-1-AP bindet in WB, IHC, IF/ICC, ELISA OS9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OS9 fusion protein Ag0106
• Vollständiger Name: osteosarcoma amplified 9, endoplasmic reticulum associated protein
• Berechnetes Molekulargewicht: 76 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC000532
• Gene symbol: OS9
• Gene ID (NCBI): 10956
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors. Isoform 2 is the major isoform detected in all cell types examined. This antibody can recognize all three isoforms of OS-9.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; OS-9 and GRP94 deliver mutant alpha1-antitrypsin to the Hrd1-SEL1L ubiquitin ligase complex for ERAD.; Authors - Christianson John C JC ; KD Validated
Mouse	WB	Sci Adv; Adult-onset obesity is triggered by impaired mitochondrial gene expression.; Authors - Kara L Perks
human	WB,IF	J Biol Chem; OS-9 regulates the transit and polyubiquitination of TRPV4 in the endoplasmic reticulum.; Authors - Wang Yan Y ; KD Validated
human	WB	J Biol Chem; Human OS-9, a lectin required for glycoprotein endoplasmic reticulum-associated degradation, recognizes mannose-trimmed N-glycans.; Authors - Hosokawa Nobuko N
human	WB	J Biol Chem; Human XTP3-B forms an endoplasmic reticulum quality control scaffold with the HRD1-SEL1L ubiquitin ligase complex and BiP.; Authors - Hosokawa Nobuko N
human	WB	J Biol Chem; SEL1L protein critically determines the stability of the HRD1-SEL1L endoplasmic reticulum-associated degradation (ERAD) complex to optimize the degradation kinetics of ERAD substrates.; Authors - Iida Yasutaka Y ; KD Validated

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