OS9 Rekombinanter Antikörper

OS9 Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230149C6

Kat-Nr. : 82932-2-PBS

Synonyme

230149C6, Amplified in osteosarcoma 9, Protein OS-9

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  IHC staining of mouse testis using 82932-2-RR (same clone as 82932-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-2-RR (OS9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82932-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 82932-2-RR (same clone as 82932-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82932-2-RR (OS9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82932-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82932-2-RR (same clone as 82932-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OS9 antibody (82932-2-RR, Clone: 230149C6 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 82932-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82932-2-RR (same clone as 82932-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug OS9 Recombinant antibody (82932-2-RR, Clone:230149C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82932-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82932-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82932-2-RR against Human OS9 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82932-2-PBS bindet in IHC, IF/ICC, FC (Intra), Indirect ELISA OS9 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: OS9 fusion protein Ag0106
• Vollständiger Name: osteosarcoma amplified 9, endoplasmic reticulum associated protein
• Berechnetes Molekulargewicht: 76 kDa
• GenBank-Zugangsnummer: BC000532
• Gene symbol: OS9
• Gene ID (NCBI): 10956
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors.