Oncostatin M Polyklonaler Antikörper

Oncostatin M Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 27792-1-AP

Synonyme

OSM, Oncostatin-M

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  WB analysis of MCF-7 using 27792-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27792-1-AP (Oncostatin M antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 27792-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27792-1-AP (Oncostatin M antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 27792-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 27792-1-AP (Oncostatin M antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 27792-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27792-1-AP (Oncostatin M antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27792-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27792-1-AP (Oncostatin M antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 27792-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27792-1-AP (Oncostatin M antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 27792-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27792-1-AP (Oncostatin M antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, Jurkat-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

27792-1-AP bindet in WB, IHC, IF, ELISA Oncostatin M und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Oncostatin M fusion protein Ag27093
• Vollständiger Name: oncostatin M
• Berechnetes Molekulargewicht: 252 aa, 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC011589
• Gene symbol: OSM
• Gene ID (NCBI): 5008
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Oncostatin M (OSM) is a multifunctional cytokine that belongs to the interleukin-6 (IL-6) family. It is primarily secreted by activated T lymphocytes and macrophages as a 28 kDa glycoprotein. OSM shares properties with all members of this cytokine family, but is most closely related in structure and function to leukemia inhibitory factor (LIF). OSM acts on a variety of cells and is involved in regulating gene activation, cell growth, and inflammatory processes. It is a potent inducer of anti-proteases, has anti-inflammatory properties, acts as an immune modifier, and promotes the deposition of extracellular matrix proteins . In the context of respiratory diseases, OSM may play a significant role in normal wound healing, as well as in remodeling and pathological fibrosis.

Publikationen

Species	Application	Title
human	IF	Jpn J Ophthalmol; Role of oncostatin M in the pathogenesis of vernal keratoconjunctivitis: focus on tissue remodeling.; Authors - Keitaro Mashimo
mouse	IHC	Int J Radiat Oncol Biol Phys; Oncostatin M/Oncostatin M Receptor Signal Induces Radiation-Induced Heart Fibrosis by Regulating SMAD4 in Fibroblast; Authors - Peng Xu
human	WB,IHC	Neoplasia; β-Catenin mediated TAM phenotype promotes pancreatic cancer metastasis via the OSM/STAT3/LOXL2 axis; Authors - Yijia Zhang