OTC Monoklonaler Antikörper

OTC Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F11C7

Kat-Nr. : 67553-1-PBS

Synonyme

OCTD, ornithine carbamoyltransferase, Ornithine transcarbamylase, OTC, OTCase

---

• 

  WB analysis using 67553-1-Ig (same clone as 67553-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67553-1-Ig (OTC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67553-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67553-1-Ig (same clone as 67553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67553-1-Ig (OTC antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67553-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 67553-1-Ig (same clone as 67553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67553-1-Ig (OTC antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67553-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67553-1-Ig (same clone as 67553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67553-1-Ig (OTC antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67553-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67553-1-PBS bindet in WB, IHC, Indirect ELISA OTC und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: OTC fusion protein Ag24186
• Vollständiger Name: ornithine carbamoyltransferase
• Berechnetes Molekulargewicht: 354 aa, 40 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC074745
• Gene symbol: OTC
• Gene ID (NCBI): 5009
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ornithine transcarbamylase (OTC) is a liver and intestinal mucosa-located, intramitochondrial, rate-limiting enzyme in the urea cycle. It catalyzes the reaction that converts ornithine and carbamoyl phosphate into citrulline, which is the second step of the urea cycle, to detoxify the ammonia produced from amino acid catabolism.