OTUB1 Polyklonaler Antikörper

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• KD/KO Validated

OTUB1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Rezensionen (1)

Kat-Nr. : 10573-1-AP

Synonyme

EC:3.4.19.12, hOTU1, HSPC263, OTB1, OTU domain-containing ubiquitin aldehyde-binding protein 1

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  WB analysis of HepG2 using 10573-1-AP

  WB result of OTUB1 antibody (10573-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTUB1 transfected HepG2 cells.

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  WB analysis using 10573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10573-1-AP (OTUB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10573-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10573-1-AP (OTUB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 10573-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using OTUB1 antibody (10573-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HepG2-Zellen, Maushirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

10573-1-AP bindet in WB, IF/ICC, IP, CoIP, RIP, ELISA OTUB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OTUB1 fusion protein Ag0845
• Vollständiger Name: OTU domain, ubiquitin aldehyde binding 1
• Berechnetes Molekulargewicht: 31 kDa and 35 kDa
• Beobachtetes Molekulargewicht: 31-35 kDa
• GenBank-Zugangsnummer: BC007519
• Gene symbol: OTUB1
• Gene ID (NCBI): 55611
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OTUB1 belongs to the ovarian tumor domain protease (OTU) subfamily of deubiquitinases, which could block ubiquitination leading to protein stability. OTUB1 mainly prefers k-48 and k-63 ubiquitin chains type, especially in k-48 ubiquitin chain. OTUB1 performs its deubiquitinating function via two distinct manners: the canonical and non-canonical manner. OTUB1 is involved in many critical immune response processes, containing CD8 T cell activation, PD-L1 related immune evasion, and several infectious diseases. OTUB1 plays an important role in the occurrence, proliferation, invasion, and progression of various cancers, such as bladder cancer, breast cancer, prostate cancer, and colorectal cancer (PMID: 34875341, 35296795). OTUB1 has 2 isoforms with molecular weights of 31 and 35 kDa, respectively. With phospho or ubiquitin modification or reaction, the MW of OTUB1 will be increased.

Publikationen

Species	Application	Title
human	RIP	Adv Sci (Weinh); Primary Cilia Formation Mediated by Hsa_Circ_0005185/OTUB1/RAB8A Complex Inhibits Prostate Cancer Progression by Suppressing Hedgehog Signaling Pathway; Authors - Aoyu Fan
mouse	WB,IP,IF	Biochem Pharmacol; OTUB1 enhances fatty acid oxidation in APAP-induced liver injury by mediating ACSL5 deubiquitination; Authors - Shuyu Guo
mouse	WB	iScience; Deacetylation Inhibition Reverses PABPN1-Dependent Muscle Wasting.; Authors - Cyriel S Olie
human	WB	ACS Pharmacol Transl Sci; Bruceine B Displays Potent Antimyeloma Activity by Inducing the Degradation of the Transcription Factor c-Maf; Authors - Hongyue Li
mouse	WB,IF	Exp Cell Res; OTUB1 mediates PARP1 deubiquitination to alleviate NAFLD by regulating HMGB1; Authors - Shuhua Ai
	CoIP	Sci Rep; CircSCD1 inhibits ferroptosis in breast Cancer through stabilizing SCD1 protein via deubiquitinase OTUB1; Authors - Zhiwei Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angie (Verified Customer) (04-09-2024)

The antibody detected a band between 25 and 37 kD at dilution of 1:3000 incubated at room temperature for 2 hours. This is followed by secondary antibody donkey-anti-rabbit (Alexa Fluor 800) used at 1:20000 dilution and incubated for 1 hour at room temperature.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: A549, HEK293

OTUB1 Antibody Western Blot validation (1:3000 dilution) in A549, HEK293 (Cat no:10573-1-AP)