OTUB1 Monoklonaler Antikörper

OTUB1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F7F8

Kat-Nr. : 68489-1-Ig

Synonyme

Deubiquitinating enzyme OTUB1, FLJ20113, FLJ40710, hOTU1, HSPC263, OTB1, OTU1, OTUB1, Otubain 1, Ubiquitin thioesterase OTUB1

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Galerie der Validierungsdaten

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  WB analysis using 68489-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68489-1-Ig (OTUB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68489-1-Ig bindet in WB, ELISA OTUB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: OTUB1 fusion protein Ag33132
• Vollständiger Name: OTU domain, ubiquitin aldehyde binding 1
• Berechnetes Molekulargewicht: 31 kDa and 35 kDa
• Beobachtetes Molekulargewicht: 31-35 kDa
• GenBank-Zugangsnummer: BC007519
• Gene symbol: OTUB1
• Gene ID (NCBI): 55611
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OTUB1 belongs to the ovarian tumor domain protease (OTU) subfamily of deubiquitinases, which could block ubiquitination leading to protein stability. OTUB1 mainly prefers k-48 and k-63 ubiquitin chains type, especially in k-48 ubiquitin chain. OTUB1 performs its deubiquitinating function via two distinct manners: the canonical and non-canonical manner. OTUB1 is involved in many critical immune response processes, containing CD8 T cell activation, PD-L1 related immune evasion, and several infectious diseases. OTUB1 plays an important role in the occurrence, proliferation, invasion, and progression of various cancers, such as bladder cancer, breast cancer, prostate cancer, and colorectal cancer (PMID: 34875341, 35296795). OTUB1 has 2 isoforms with molecular weights of 31 and 35 kDa, respectively.