OTUB1 Monoklonaler Antikörper

OTUB1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F7F8

Kat-Nr. : 68489-1-PBS

Synonyme

OTU domain-containing ubiquitin aldehyde-binding protein 1, OTB1, HSPC263, hOTU1, EC:3.4.19.12

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  WB analysis using 68489-1-Ig (same clone as 68489-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68489-1-Ig (OTUB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68489-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 68489-1-Ig (same clone as 68489-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using OTUB1 antibody (68489-1-Ig, Clone: 2F7F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68489-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68489-1-PBS bindet in WB, IF/ICC, Indirect ELISA OTUB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: OTUB1 fusion protein Ag33132
• Vollständiger Name: OTU domain, ubiquitin aldehyde binding 1
• Berechnetes Molekulargewicht: 31 kDa and 35 kDa
• Beobachtetes Molekulargewicht: 31-35 kDa
• GenBank-Zugangsnummer: BC007519
• Gene symbol: OTUB1
• Gene ID (NCBI): 55611
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OTUB1 belongs to the ovarian tumor domain protease (OTU) subfamily of deubiquitinases, which could block ubiquitination leading to protein stability. OTUB1 mainly prefers k-48 and k-63 ubiquitin chains type, especially in k-48 ubiquitin chain. OTUB1 performs its deubiquitinating function via two distinct manners: the canonical and non-canonical manner. OTUB1 is involved in many critical immune response processes, containing CD8 T cell activation, PD-L1 related immune evasion, and several infectious diseases. OTUB1 plays an important role in the occurrence, proliferation, invasion, and progression of various cancers, such as bladder cancer, breast cancer, prostate cancer, and colorectal cancer (PMID: 34875341, 35296795). OTUB1 has 2 isoforms with molecular weights of 31 and 35 kDa, respectively.