OTUD1 Polyklonaler Antikörper

OTUD1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29921-1-PBS

Synonyme

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  WB analysis using 29921-1-AP (same clone as 29921-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 29921-1-AP (same clone as 29921-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29921-1-AP (same clone as 29921-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 29921-1-AP (same clone as 29921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 29921-1-AP (same clone as 29921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 29921-1-AP (same clone as 29921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 29921-1-AP (same clone as 29921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 29921-1-AP (same clone as 29921-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29921-1-PBS bindet in WB, IHC, Indirect ELISA OTUD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OTUD1 fusion protein Ag31996
• Vollständiger Name: OTU domain containing 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• Gene symbol: OTUD1
• Gene ID (NCBI): 220213
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OTUD1, OTU domain-containing protein 1, is a deubiquitinating enzyme that specifically hydrolyzes 'Lys-63'-linked polyubiquitin to monoubiquitin (PMID: 23827681). OTUD1-mediated deubiquitination prevents activation of the ribosome quality control and subsequent dissociation of ribosomes and stimulates formation of polysomes and translation (PMID: 36445135). OTUD1 is a colon-specific deubiquitinase that expressed in normal colonic epithelium goblet cells (PMID: 36910614).