OTUD3 Polyklonaler Antikörper

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OTUD3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 29622-1-AP

Synonyme

DUBA4, KIAA0459, OTU domain containing 3, RP11 460G22.1

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  WB analysis of HeLa using 29622-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29622-1-AP (OTUD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HCT 116 using 29622-1-AP

  WB result of OTUD3 antibody (29622-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTUD3 transfected HCT 116 cells.

• 

  WB analysis using 29622-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29622-1-AP (OTUD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 29622-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29622-1-AP (OTUD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 29622-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29622-1-AP (OTUD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HCT 116-Zellen, HepG2-Zellen, HT-29-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

29622-1-AP bindet in WB, IHC, ELISA OTUD3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OTUD3 fusion protein Ag30425
• Vollständiger Name: OTU domain containing 3
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: NM_015207
• Gene symbol: OTUD3
• Gene ID (NCBI): 23252
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OTUD3 is a kind of deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It's an important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation. The calculated MW of OTUD3 is 45 kDa, 29622-1-AP can detect the band around 43 kDa. (PubMed:23827681, 35675826).

Publikationen

Species	Application	Title
human	WB	Nat Commun; Combinatorial ubiquitin code degrades deubiquitylation-protected substrates; Authors - Mai Morita
pig		Sci China Life Sci; CRISPR screening identifies protein methylation and ubiquitination modifications that modulate aflatoxin B1 cytotoxicity; Authors - Huaqiang Yang