OTUD3 Polyklonaler Antikörper

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• KD/KO Validated

OTUD3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29622-1-PBS

Synonyme

DUBA4, KIAA0459, OTU domain containing 3, RP11 460G22.1

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  WB analysis of HeLa using 29622-1-AP (same clone as 29622-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29622-1-AP (OTUD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29622-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 29622-1-AP (same clone as 29622-1-PBS)

  WB result of OTUD3 antibody (29622-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTUD3 transfected HCT 116 cells. This data was developed using the same antibody clone with 29622-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29622-1-AP (same clone as 29622-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29622-1-AP (OTUD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29622-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 29622-1-AP (same clone as 29622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29622-1-AP (OTUD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29622-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 29622-1-AP (same clone as 29622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29622-1-AP (OTUD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29622-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29622-1-PBS bindet in WB, IHC, Indirect ELISA OTUD3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OTUD3 fusion protein Ag30425
• Vollständiger Name: OTU domain containing 3
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: NM_015207
• Gene symbol: OTUD3
• Gene ID (NCBI): 23252
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OTUD3 is a kind of deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It's an important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation. The calculated MW of OTUD3 is 45 kDa, 29622-1-AP can detect the band around 43 kDa. (PubMed:23827681, 35675826).