OTUD3 Rekombinanter Antikörper

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• KD/KO Validated

OTUD3 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242202B11

Kat-Nr. : 84717-1-PBS

Synonyme

242202B11, DUBA4, KIAA0459, OTU domain containing 3, RP11 460G22.1

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• 

  WB analysis of HeLa using 84717-1-RR (same clone as 84717-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 84717-1-RR (OTUD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84717-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 84717-1-RR (same clone as 84717-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 84717-1-RR (OTUD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84717-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HCT 116 using 84717-1-RR (same clone as 84717-1-PBS)

  WB result of OTUD3 antibody (84717-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTUD3 transfected HCT 116 cells. This data was developed using the same antibody clone with 84717-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 84717-1-RR (same clone as 84717-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84717-1-RR (OTUD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84717-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01521-2

  Cytometric bead array standard curve of MP01521-2, OTUD3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84717-1-PBS. Detection antibody: 84717-2-PBS. Standard: Ag30425. Range: 0.781-50 ng/mL.

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  Affinity and Kinetic Characterization of 84717-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84717-1-RR against Human OTUD3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84717-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA OTUD3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: OTUD3 fusion protein Ag30425
• Vollständiger Name: OTU domain containing 3
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: NM_015207
• Gene symbol: OTUD3
• Gene ID (NCBI): 23252
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OTUD3 is a kind of deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It's an important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation.