OTUD3 Rekombinanter Antikörper

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• KD/KO Validated

OTUD3 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242202B11

Kat-Nr. : 84717-1-RR

Synonyme

242202B11, DUBA4, KIAA0459, OTU domain containing 3, RP11 460G22.1

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  WB analysis of HeLa using 84717-1-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 84717-1-RR (OTUD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 84717-1-RR

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 84717-1-RR (OTUD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HCT 116 using 84717-1-RR

  WB result of OTUD3 antibody (84717-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTUD3 transfected HCT 116 cells.

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  IHC staining of mouse cerebellum using 84717-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84717-1-RR (OTUD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84717-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84717-1-RR against Human OTUD3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HCT 116-Zellen, HepG2-Zellen, MCF-7-Zellen, Neuro-2a-Zellen
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84717-1-RR bindet in WB, IHC, ELISA OTUD3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: OTUD3 fusion protein Ag30425
• Vollständiger Name: OTU domain containing 3
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: NM_015207
• Gene symbol: OTUD3
• Gene ID (NCBI): 23252
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OTUD3 is a kind of deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It's an important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation.