OTUD4 Polyklonaler Antikörper

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• KD/KO Validated

OTUD4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Kat-Nr. : 25070-1-AP

Synonyme

DUBA6, HIN1, HIV 1 induced protein HIN 1, HSHIN1, KIAA1046, OTU domain containing 4, OTUD4

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  WB analysis using 25070-1-AP

  Various lysate were subjected to SDS PAGE followed by western blot with 25070-1-AP (OTUD4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 25070-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25070-1-AP (OTUD4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 25070-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25070-1-AP (OTUD4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Produktinformation

25070-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA OTUD4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OTUD4 fusion protein Ag18401
• Vollständiger Name: OTU domain containing 4
• Berechnetes Molekulargewicht: 1113 aa, 124 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC118653
• Gene symbol: OTUD4
• Gene ID (NCBI): 54726
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OTUD4, a member of the OTU family, encodes for a protein of 495 amino acids with an OTU domain and functions via their enzymatic deubiquitinating activities. OTUD4 nominally encodes a K48-specific deubiquitinase previously shown to counteract proteasomal degradation of DNA repair proteins. OTUD4 has been identified to be down-regulated in multiple human tumor types, and lower OTUD4 expression indicates a poor prognosis.(PMID: 36411454,PMID: 32184655,PMID: 29395066)

Publikationen

Species	Application	Title
human	WB	Nat Commun; Combinatorial ubiquitin code degrades deubiquitylation-protected substrates; Authors - Mai Morita
mouse	WB	Nat Commun; Osteocyte-derived extracellular vesicles mediate the bone-to-cartilage crosstalk and promote osteoarthritis progression; Authors - Na Liu
human	WB	Adv Sci (Weinh); Elevated SPARC Disrupts the Intestinal Barrier Integrity in Crohn's Disease by Interacting with OTUD4 and Activating the MYD88/NF-κB Pathway; Authors - Jiayu Wang
human	WB	Cell Death Differ; N6-methyladenosine-modified USP13 induces pro-survival autophagy and imatinib resistance via regulating the stabilization of autophagy-related protein 5 in gastrointestinal stromal tumors; Authors - Zhishuang Gao
human	WB	Front Microbiol; MiR-103a-3p Promotes Zika Virus Replication by Targeting OTU Deubiquitinase 4 to Activate p38 Mitogen-Activated Protein Kinase Signaling Pathway.; Authors - Haiyan Ye ; KD Validated
human	WB,IHC,IF,IP	Exp Cell Res; Deubiquitinating enzyme OTUD4 regulates metastasis in triple-negative breast cancer by stabilizing Snail1; Authors - Xiuqing Ma ; KD Validated