OTUD5 Polyklonaler Antikörper

OTUD5 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 21002-1-AP

Synonyme

OTUD 5, OTU domain-containing protein 5, EC:3.4.19.12, DUBA

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  WB analysis using 21002-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21002-1-AP (OTUD5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21002-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21002-1-AP (OTUD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 21002-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using OTUD5 antibody (21002-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, C6-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

21002-1-AP bindet in WB, IF/ICC, IP, CoIP, ELISA OTUD5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OTUD5 fusion protein Ag15190
• Vollständiger Name: OTU domain containing 5
• Berechnetes Molekulargewicht: 571 aa, 61 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC028225
• Gene symbol: OTUD5
• Gene ID (NCBI): 55593
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The deubiquitinating enzyme OTUD5, also known as deubiquitinating enzyme A (DUBA), is a member of the deubiquitinating enzyme OTU family. By deubiquitinating other proteins, OTUD5 is an important regulator of several physiological processes, such as immune signaling and DNA damage responses. The dysregulation of these processes can increase the risk of associated diseases, including inflammation, cancer and genetic disorders.

Publikationen

Species	Application	Title
human,mouse	WB,IP,IF	Nat Commun; OTUD5 promotes end-joining of deprotected telomeres by promoting ATM-dependent phosphorylation of KAP1S824; Authors - Shiu Yeung Lam
human,mouse	WB,IF	Adv Sci (Weinh); OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model; Authors - Xiaomeng Song
human	WB,IF	Clin Transl Med; p53 inhibits OTUD5 transcription to promote GPX4 degradation and induce ferroptosis in gastric cancer; Authors - Junjing Zhang ; KD Validated
human,mouse	WB	Int J Radiat Oncol Biol Phys; USP11 exacerbates radiation induced pneumonitis by activating endothelial cell inflammatory response via OTUD5-STING signaling; Authors - Yiting Tang
human	WB	Carcinogenesis; CacyBP promotes the development of lung adenocarcinoma by regulating OTUD5; Authors - Mixue Bai
mouse	WB,IF,CoIP	Nat Commun; Podocyte OTUD5 alleviates diabetic kidney disease through deubiquitinating TAK1 and reducing podocyte inflammation and injury; Authors - Ying Zhao