OTUD6B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

OTUD6B Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 25430-1-AP

Synonyme

DUBA-5, DUBA5, DUBA 5, Deubiquitinase OTUD6B, CGI-77

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  WB analysis using 25430-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25430-1-AP (OTUD6B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25430-1-AP

  WB result of OTUD6B antibody (25430-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTUD6B transfected HeLa cells.

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  WB analysis of HEK-293 using 25430-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25430-1-AP (OTUD6B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 25430-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25430-1-AP (OTUD6B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25430-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25430-1-AP (OTUD6B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 25430-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 25430-1-AP (OTUD6B antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 25430-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 25430-1-AP (OTUD6B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

25430-1-AP bindet in WB, IHC, IF/ICC, ELISA OTUD6B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OTUD6B fusion protein Ag22044
• Vollständiger Name: OTU domain containing 6B
• Berechnetes Molekulargewicht: 293 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34-40 kDa
• GenBank-Zugangsnummer: BC029760
• Gene symbol: OTUD6B
• Gene ID (NCBI): 51633
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB	Cell Mol Life Sci; Otud6b induces pulmonary arterial hypertension by mediating the Calpain-1/HIF-1α signaling pathway; Authors - Yu Liu ; KD Validated
human	IHC,IF	Cell Biol Int; OTUD6B promotes cholangiocarcinoma growth by regulating STAT3 phosphorylation through deubiquitination of PTK2; Authors - Guoqiang Xing ; KD Validated
human	WB	EMBO Rep; OTUD6B regulates KIFC1-dependent centrosome clustering and breast cancer cell survival; Authors - Valeria E Marotta

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Dorota (Verified Customer) (04-11-2019)

Antibody works perfectly for western blotting, however I could not optimise it for immunofluorescence. I tried series of dilutions and couple of different blocking/fixing methods, but there was no difference between knockdown and control conditions in any of them.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:500-1:5000
• Cell Tissue Type: BT549

OTUD6B Antibody Western Blot,Immunofluorescence, validation (1:500-1:5000 dilution) in BT549 (Cat no:25430-1-AP)