OTUD7B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

OTUD7B Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Rezensionen (1)

Kat-Nr. : 16605-1-AP

Synonyme

CEZANNE, OTU domain containing 7B, OTU7B, OTUD7B, ZA20D1, Zinc finger protein Cezanne

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 16605-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16605-1-AP (OTUD7B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16605-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16605-1-AP (OTUD7B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16605-1-AP

  IP Result of anti-OTUD7B (IP:16605-1-AP, 4ug; Detection:16605-1-AP 1:800) with Jurkat cells lysate 2500ug.

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  IHC staining of human kidney using 16605-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 16605-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse kidney using 16605-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16605-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16605-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16605-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 16605-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 16605-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 16605-1-AP (OTUD7B antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe, Mausherzgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

16605-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA OTUD7B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OTUD7B fusion protein Ag9788
• Vollständiger Name: OTU domain containing 7B
• Berechnetes Molekulargewicht: 427aa,48 kDa; 843aa,93 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC020622
• Gene symbol: OTUD7B
• Gene ID (NCBI): 56957
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OUTD7B is a member of the A20 family of the deubiquitinating cysteine proteases, which has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. It plays a key role in the negative feedback regulation of NF-κB signaling in response to multiple stimuli and has been described as central gatekeeper in inflammation and immunity. The calculated molecular weight of OTUD7B is 93 kDa, but the modified OTUD7B is about 100 kDa.

Publikationen

Species	Application	Title
mouse	WB,IP	Nature; OTUD7B controls non-canonical NF-κB activation through deubiquitination of TRAF3.; Authors - Hu Hongbo H ; KO Validated
human	WB	Nat Commun; Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation.; Authors - Chun-Ping Cui ; KD Validated
mouse	WB	J Exp Med; Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination.; Authors - Hongbo Hu ; KO Validated
human	WB	Circ Res; Cezanne regulates inflammatory responses to hypoxia in endothelial cells by targeting TRAF6 for deubiquitination.; Authors - Luong Le A le A
mouse	IHC,WB,IF,CoIP	Theranostics; Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.; Authors - Gao-Shan Yang
mouse	WB,IF	Arterioscler Thromb Vasc Biol; Mechanical Activation of Hypoxia-Inducible Factor 1α Drives Endothelial Dysfunction at Atheroprone Sites.; Authors - Shuang Feng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Francesca (Verified Customer) (06-02-2019)

Good and specific antibody for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1: 2000 in 5% milk
• Cell Tissue Type: MeT-5A cells

OTUD7B Antibody Western Blot, validation (1: 2000 in 5% milk dilution) in MeT-5A cells (Cat no:16605-1-AP)