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OTULIN Polyklonaler Antikörper

OTULIN Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27392-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using OTULIN Polyclonal antibody (27392-1-AP)

    WB analysis using 27392-1-AP (same clone as 27392-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27392-1-AP (OTULIN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27392-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

27392-1-PBS bindet in WB, Indirect ELISA OTULIN und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen OTULIN fusion protein Ag22302
Vollständiger Name family with sequence similarity 105, member B
Berechnetes Molekulargewicht 352 aa, 40 kDa
Beobachtetes Molekulargewicht40-50 kDa
GenBank-ZugangsnummerBC007706
Gene symbol FAM105B
Gene ID (NCBI) 90268
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Linear ubiquitination is negatively regulated by OTULIN (OTU deubiquitinase with linear linkage specificity, also known as Fam105b or Gumby), a deubiquitinase specifically catalysing the degradation of linear ubiquitin chains. OUb signalling is antagonised by deubiquitinases (DUBs), which cleave the polyUb signal from substrates to terminate signalling. OTU DUB with linear linkage specificity (OTULIN) and CYLD are the two main DUBs that regulate M1-polyUb signalling. OTULIN exclusively cleaves M1 linkages, whereas CYLD cleaves both M1 and K63 linkages. OTULIN binds directly to the LUBAC subunit HOIP and regulates LUBAC signalling, autoubiquitination, and stability.(PMID: 34625557, PMID: 32231246)