OX40/TNFRSF4 Rekombinanter Antikörper

OX40/TNFRSF4 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241398C10

Kat-Nr. : 84247-1-PBS

Synonyme

TNFRSF4, ACT35, ACT35 antigen, CD134, OX40

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  Sandwich ELISA sample test of MP01138-2

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean CD134/OX40 concentration was not determined in unstimulated PBMC supernatant, 232.1 pg/mL in PHA stimulated PBMC supernatant.

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  Sandwich ELISA sample test of MP01138-2

  The mean CD134/OX40 concentration was determined to be 4.0 ng/mL in Jurkat cell extract based on a 1.5 mg/mL extract load.

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  Sandwich ELISA sample test of MP01138-2

  Serum of six individual healthy human donors was measured. The CD134/OX40 concentration of detected samples was determined to be 780.8 pg/mL with a range of 152.2-1,428.1 pg/mL.

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  Sandwich ELISA standard curve of MP01138-2

  Sandwich ELISA standard curve of MP01138-2, Human CD134/OX40 Recombinant Matched Antibody Pair - PBS only. 84247-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1261. 84247-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

• 

  Cytometric bead array standard curve of MP01138-2

  Cytometric bead array standard curve of MP01138-2, CD134/OX40 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84247-2-PBS. Detection antibody: 84247-1-PBS. Standard: Eg1261. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84247-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 84247-1-PBS against Human PROS1 was performed. The affinity constant is 6.23 nM.

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Geprüfte Anwendungen

Produktinformation

84247-1-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA OX40/TNFRSF4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: OX40/TNFRSF4 fusion protein Eg1261
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 4
• Berechnetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: NM_003327
• Gene symbol: CD134
• Gene ID (NCBI): 7293
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.