OX40L/TNFSF4 Rekombinanter Antikörper

OX40L/TNFSF4 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242227B1

Kat-Nr. : 98381-1-RR

Synonyme

CD252, OX40 ligand, OX40L, Tnfsf4, Tumor necrosis factor ligand superfamily member 4

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  FC experiment of BALB/c mouse splenocytes using 98381-1-RR

  1x10^6 untreated or Anti-IgM and CD40 treated BALB/c mouse splenocytes were surface stained with 0.25 ug Anti-Mouse OX40 Ligand/TNFSF4 Rabbit RecAb (98381-1-RR, Clone:242227B1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Mouse CD19 (1D3) (CL488-65290, Clone: 1D3). Cells were not fixed.

• 

  FC experiment of BALB/c mouse splenocytes using 98381-1-RR

  1x10^6 Anti-IgM and CD40 treated BALB/c mouse splenocytes were surface stained with 0.25 ug Anti-Mouse OX40 Ligand/TNFSF4 Rabbit RecAb (98381-1-RR, Clone:242227B1) (red) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse OX40 Ligand/TNFSF4 Rabbit RecAb (98381-1-RR, Clone:242227B1) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98381-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98381-1-RR against Mouse OX40L/TNFSF4 were performed. The affinity constant is 0.445 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-IgM and CD40 treated BALB/c mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98381-1-RR bindet in FC OX40L/TNFSF4 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: OX40L/TNFSF4 fusion protein Eg1573
• Vollständiger Name: tumor necrosis factor (ligand) superfamily, member 4
• Berechnetes Molekulargewicht: 22kDa
• GenBank-Zugangsnummer: NM_009452.2
• Gene symbol: Tnfsf4
• Gene ID (NCBI): 22164
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

The tumour necrosis factor ligand superfamily member 4 gene (TNFSF4, OX40L), which encodes for the T cell costimulatory molecule OX40 ligand, has been identified as a susceptibility gene for the development of systemic lupus erythematosus (SLE). OX40L/TNFSF4/CD252 is a co-stimulatory checkpoint protein expressed by several types of immune and non-immune cells (PMID: 15750594, 19778912). TNFSF4 mRNA is expressed in melanoma cell lines and melanoma samples, including those with low lymphocytic infiltrates, and is not associated with the ulceration status of the primary tumor (PMID: 31501955).