OXR1 Rekombinanter Antikörper

OXR1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251027B10

Kat-Nr. : 86359-3-RR

Synonyme

CHEGDD, Nbla00307, TLDC3

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  WB analysis using 86359-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86359-3-RR (OXR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 86359-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86359-3-RR (OXR1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 86359-3-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 86359-3-RR (OXR1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 86359-3-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 86359-3-RR (OXR1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 86359-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86359-3-RR (OXR1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 86359-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86359-3-RR against Human OXR1 were performed. The affinity constant is 0.533 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, K-562-Zellen, Neuro-2a-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86359-3-RR bindet in WB, IHC, ELISA OXR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: OXR1 fusion protein Ag4439
• Vollständiger Name: oxidation resistance 1
• Berechnetes Molekulargewicht: 758 aa, 85 kDa
• Beobachtetes Molekulargewicht: 120-140 kDa
• GenBank-Zugangsnummer: BC032710
• Gene symbol: OXR1
• Gene ID (NCBI): 55074
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Oxidation resistance protein 1 (OXR1) belongs to the OXR1 family. The major function of OXR1 is to control the expression of genes that alleviate oxidative stress by increasing cellular resistance to reactive oxygen species (ROS) and the stress these molecules cause the cell. OXR1's ability to reduce oxidative stress and neurodegeneration in multiple diseases strongly suggests that it can be an effective therapeutic target (PMID: 33384581). Biochemical experiments show that OXR1 inhibits V1-ATPase and causes disassembly of the holoenzyme, suggesting that OXR1 plays a direct role in V-ATPase regulation (PMID: 34918374). OXR1 has 8 isoforms with the molecular mass of 25, 28, 34, 56, 94, 95 and 98 kDa. Sometimes higher molecular weight around 120-140 kDa can also be observed, which may be a modified variant of OXR1.