OXSR1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

OXSR1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Rezensionen (1)

Kat-Nr. : 15611-1-AP

Synonyme

KIAA1101, OSR1, oxidative stress responsive 1, OXSR1

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  WB analysis using 15611-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15611-1-AP (OXSR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15611-1-AP

  WB result of OXSR1 antibody (15611-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXSR1 transfected HeLa cells.

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  WB analysis of human liver using 15611-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15611-1-AP (OXSR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15611-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15611-1-AP (OXSR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15611-1-AP

  IP result of anti-OXSR1 (IP:15611-1-AP, 4ug; Detection:15611-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IP experiment of HEK-293 using 15611-1-AP

  IP result of anti-OXSR1 (IP:15611-1-AP, 4ug; Detection:15611-1-AP 1:300) with HEK-293 cells lysate 2320 ug.

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  IHC staining of human prostate cancer using 15611-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15611-1-AP (OXSR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15611-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15611-1-AP (OXSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 15611-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using OXSR1 antibody (15611-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Lebergewebe, humanes Hodengewebe, Jurkat-Zellen, U-251-Zellen
• Erfolgreiche IP: HeLa-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15611-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA OXSR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OXSR1 fusion protein Ag8002
• Vollständiger Name: oxidative-stress responsive 1
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC008726
• Gene symbol: OXSR1
• Gene ID (NCBI): 9943
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Oxidative-stress responsive 1(OXSR1) is also named as KIAA1101, OSR1 and belongs to the STE Ser/Thr protein kinase family. It contains an N-terminal Ste20-like ser/thr kinase domain and 2 C-terminal regions, which has a putative caspase-3 cleavage site at the end. OXSR1's interaction with WNK1 is required for NKCC function, and it modulates the G protein sensitivity of PAK by phosphorylation of PAK1.Western blot analysis detected Oxsr1 at an apparent molecular mass of 58 kD in all mouse tissues examined except thymus. Cell fractionation and immunofluorescence analysis of HeLa cells showed that OXSR1 was distributed throughout the cell and OXSR1 could phosphorylate a test substrate and itself(PMID:14707132).

Publikationen

Species	Application	Title
human	IHC,WB	Bioengineered; Upregulation of Oxidative stress-responsive 1(OXSR1) Predicts Poor Prognosis and Promotes Hepatocellular Carcinoma Progression.; Authors - Jianhui Chen
rat	WB,IF	Adv Sci (Weinh); Modulation of Cerebrospinal Fluid Dysregulation via a SPAK and OSR1 Targeted Framework Nucleic Acid in Hydrocephalus; Authors - Qiguang Wang
rat	WB	Exp Mol Pathol; Proteomic analysis of the effects of Dictyophora polysaccharide on arsenic-induced hepatotoxicity in rats; Authors - Xi Yan
rat	WB	Heliyon; Sevoflurane-induced regulation of NKCC1/KCC2 phosphorylation through activation of Spak/OSR1 kinase and cognitive impairment in ischemia-reperfusion injury in rats; Authors - Yuefeng Wang
human	WB	Nat Commun; Thermal proteome profiling reveals fructose-1,6-bisphosphate as a phosphate donor to activate phosphoglycerate mutase 1; Authors - Yanling Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jennifer (Verified Customer) (03-23-2022)

Image provided from optimisation plate for OXSR1 for rat brain lysates. Analysed using Simple Western Analysis (WES) by Protein Simple. Finalised antibody and protein concentration for samples produced a single band with no non-specific binding.

• Applications: Other
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Rat and mouse brain lysate

OXSR1 Antibody Other validation (1:100 dilution) in Rat and mouse brain lysate (Cat no:15611-1-AP)