OXT Polyklonaler Antikörper

OXT Polyklonal Antikörper für IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 18041-1-AP

Synonyme

Neurophysin 1, Ocytocin Neurophysin 1, Oxytocin, Oxytocin neurophysin 1, Oxytocin-neurophysin 1

---

• 

  IP experiment of mouse pituitary gland using 18041-1-AP

  IP result of anti-OXT (IP:18041-1-AP, 4ug; Detection:18041-1-AP 1:300) with mouse pituitary gland tissue lysate 1440 ug.

• 

  IHC staining of human pituitary using 18041-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pituitary using 18041-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18041-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 18041-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 18041-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 18041-1-AP (OXT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche IP: mouse pituitary gland tissue
• Erfolgreiche Detektion in IHC: humanes Hypophysengewebe, Maushirngewebe, Maus-Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

18041-1-AP bindet in IHC, IF, IP, ELISA OXT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OXT fusion protein Ag12672
• Vollständiger Name: oxytocin, prepropeptide
• Berechnetes Molekulargewicht: 125 aa, 13 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC101841
• Gene symbol: OXT
• Gene ID (NCBI): 5020
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Oxytocin (OXT) is a neuropeptide involved in social-approach behaviors in humans and others mammals (PMID: 27325757). OXT is located on chromosome 20p13 and codes for a precursor protein that is synthetized to produce oxytocin and neurophysin I, and neurophysin I is the carrier protein for oxytocin (PMID: 1486803, PMID: 18566739). Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Acts by binding to oxytocin receptor (OXTR) (PMID: 18174156).

Publikationen

Species	Application	Title
mouse	IF	Front Endocrinol (Lausanne); Targeted deletion of the pancreatic β-cell oxytocin receptor and its effects on metabolic regulation and β-cell health; Authors - Armando J Mendez