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OXTR Polyklonaler Antikörper
OXTR Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (3)
Anwendung
WB, IHC, IF/ICC, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 23045-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Jurkat-Zellen, HeLa-Zellen, L02-Zellen |
| Erfolgreiche IP | L02-Zellen |
| Erfolgreiche Detektion in IHC | Maus-Plazenta-Gewebe, humanes Mammakarzinomgewebe, humanes Uterusmyomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:200-1:800 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 3 publications below |
| WB | See 11 publications below |
| IHC | See 11 publications below |
| IF | See 8 publications below |
| IP | See 1 publications below |
Produktinformation
23045-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA OXTR und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | OXTR fusion protein Ag19074 |
| Vollständiger Name | OXTR |
| Berechnetes Molekulargewicht | 389 aa, 43 kDa |
| Beobachtetes Molekulargewicht | 46 kDa, 67 kDa |
| GenBank-Zugangsnummer | BC137443 |
| Gene symbol | OXTR |
| Gene ID (NCBI) | 5021 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Oxytocin (OXT) is a neurohypophysial hormone that plays a role in lactation and parturition and in the central nervous system as a neurotransmitter involved in sex and maternal behavior (PMID: 1852313; 10811917). OXT acts through its receptor, OXTR, which belongs to the G protein-coupled 7-transmembrane receptor family. OXTR is expressed in the endometrium, myometrium, and mammary gland (PMID: 1313946). This antibody is raised against the C-terminal region of human OXTR. Two immunoreactive OXTR bands were found in the fat tissue, an unglycosylated 43 kDa form and a mature glycosylated 67 kDa form (PMID:32819381).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for OXTR antibody 23045-1-AP | Protokoll herunterladen |
| IHC protocol for OXTR antibody 23045-1-AP | Protokoll herunterladenl |
| IF protocol for OXTR antibody 23045-1-AP | Protokoll herunterladen |
| IP protocol for OXTR antibody 23045-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Acta Physiol (Oxf) Oxytocin modulates neural processing of mitral/tufted cells in the olfactory bulb. | ||
Antioxidants (Basel) ΔMST and the Regulation of Cardiac CSE and OTR Expression in Trauma and Hemorrhage. | ||
Biochem Pharmacol Synergistic antitumor activity of 5-fluorouracil and atosiban against microsatellite stable colorectal cancer through restoring GATA3.
| ||
Front Pharmacol The Role of Formyl Peptide Receptor 1 in Uterine Contraction During Parturition. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Rachael (Verified Customer) (08-29-2025) | Ran it through the protein simple abby machine and the results was a weak peak with lots of background noise, which meant we couldn't optimise and run our samples. when using it in immunofluorescence there was a lot of background staining
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