OXTR Polyklonaler Antikörper

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• KD/KO Validated

OXTR Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(24)

Rezensionen (1)

Kat-Nr. : 23045-1-AP

Synonyme

OT R, OT-R, Oxytocin receptor

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  WB analysis using 23045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23045-1-AP (OXTR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23045-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23045-1-AP (OXTR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23045-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23045-1-AP (Oxytocin Receptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 23045-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 23045-1-AP (Oxytocin Receptor antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 23045-1-AP

  IP result of anti-OXTR (IP:23045-1-AP, 4ug; Detection:23045-1-AP 1:1000) with L02 cells lysate 1800ug.

• 

  IHC staining of mouse placenta using 23045-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 23045-1-AP (OXTR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hysteromyoma using 23045-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23045-1-AP (OXTR antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hysteromyoma using 23045-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23045-1-AP (OXTR antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 23045-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 23045-1-AP (Oxytocin Receptor antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 23045-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 23045-1-AP (Oxytocin Receptor antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 23045-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using OXTR antibody (23045-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, L02-Zellen
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IHC: Maus-Plazenta-Gewebe, humanes Mammakarzinomgewebe, humanes Uterusmyomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 11 publications below
• IF: See 8 publications below
• IP: See 1 publications below

Produktinformation

23045-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA OXTR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OXTR fusion protein Ag19074
• Vollständiger Name: OXTR
• Berechnetes Molekulargewicht: 389 aa, 43 kDa
• Beobachtetes Molekulargewicht: 46 kDa, 67 kDa
• GenBank-Zugangsnummer: BC137443
• Gene symbol: OXTR
• Gene ID (NCBI): 5021
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Oxytocin (OXT) is a neurohypophysial hormone that plays a role in lactation and parturition and in the central nervous system as a neurotransmitter involved in sex and maternal behavior (PMID: 1852313; 10811917). OXT acts through its receptor, OXTR, which belongs to the G protein-coupled 7-transmembrane receptor family. OXTR is expressed in the endometrium, myometrium, and mammary gland (PMID: 1313946). This antibody is raised against the C-terminal region of human OXTR. Two immunoreactive OXTR bands were found in the fat tissue, an unglycosylated 43 kDa form and a mature glycosylated 67 kDa form (PMID:32819381).

Publikationen

Species	Application	Title
mouse	WB,IF	Nat Commun; PMID: 24915299; Authors - Christian Elabd
mouse	IF	Acta Physiol (Oxf); Oxytocin modulates neural processing of mitral/tufted cells in the olfactory bulb.; Authors - Changcheng Sun
mouse	IF	Oxid Med Cell Longev; PMID: 32082478; Authors - Daniela C Wigger
mouse	IHC	Antioxidants (Basel); ΔMST and the Regulation of Cardiac CSE and OTR Expression in Trauma and Hemorrhage.; Authors - Britta Trautwein
human	WB	Biochem Pharmacol; Synergistic antitumor activity of 5-fluorouracil and atosiban against microsatellite stable colorectal cancer through restoring GATA3.; Authors - Mengmeng Wang ; KD Validated
human	WB	Front Pharmacol; The Role of Formyl Peptide Receptor 1 in Uterine Contraction During Parturition.; Authors - Chaolu Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rachael (Verified Customer) (08-29-2025)

Ran it through the protein simple abby machine and the results was a weak peak with lots of background noise, which meant we couldn't optimise and run our samples. when using it in immunofluorescence there was a lot of background staining

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Cell Tissue Type: rat brain