Occludin Monoklonaler Antikörper

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Occludin Monoklonal Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D3C4

Kat-Nr. : 66378-1-PBS

Synonyme

OCLN, 1D3C4

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  WB analysis using 66378-1-Ig (same clone as 66378-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66378-1-Ig (same clone as 66378-1-PBS)

  WB result of Occludin antibody (66378-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Occludin transfected Jurkat cells. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 66378-1-Ig (same clone as 66378-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66378-1-Ig (same clone as 66378-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig colon using 66378-1-Ig (same clone as 66378-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66378-1-Ig (same clone as 66378-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66378-1-Ig (Occludin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66378-1-Ig (same clone as 66378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66378-1-Ig (same clone as 66378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66378-1-Ig (same clone as 66378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66378-1-Ig (same clone as 66378-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66378-1-Ig (Occludin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66378-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP51160-1

  Cytometric bead array standard curve of MP51160-1, Occludin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66378-2-PBS. Detection antibody: 66378-1-PBS. Standard:Ag4057. Range: 0.098-100 ng/mL.

• 

  Cytometric bead array standard curve of MP51160-3

  Cytometric bead array standard curve of MP51160-3, Occludin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66378-1-PBS. Detection antibody: 66378-4-PBS. Standard:Ag4057. Range: 0.098-50 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66378-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA Occludin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Occludin fusion protein Ag4057
• Vollständiger Name: occludin
• Berechnetes Molekulargewicht: 522 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC029886
• Gene symbol: Occludin
• Gene ID (NCBI): 4950
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).