Occludin Rekombinanter Antikörper

Occludin Rekombinant Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

8B2

Kat-Nr. : 80545-1-PBS

Synonyme

OCLN, 8B2

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  WB analysis using 80545-1-RR (same clone as 80545-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80545-1-RR (same clone as 80545-1-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig colon using 80545-1-RR (same clone as 80545-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 80545-1-RR (same clone as 80545-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 80545-1-RR (Occludin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HUVEC using 80545-1-RR (same clone as 80545-1-PBS)

  IP result of anti-Occludin (IP:80545-1-RR, 4ug; Detection:80545-1-RR 1:15000) with HUVEC cells lysate 1410 ug. This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 80545-1-RR (same clone as 80545-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Occludin antibody (80545-1-RR, Clone: 8B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 80545-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 80545-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 80545-1-RR against Human Occludin were performed. The affinity constant is 26.5 pM.

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Geprüfte Anwendungen

Produktinformation

80545-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA Occludin und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Occludin fusion protein Ag26173
• Vollständiger Name: occludin
• Berechnetes Molekulargewicht: 522 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC029886
• Gene symbol: Occludin
• Gene ID (NCBI): 4950
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).