Occludin Polyklonaler Antikörper

Occludin Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(3)

Kat-Nr. : CL488-27260

Synonyme

OCLN

---

• 

  IF Staining of MCF-7 using CL488-27260

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 Occludin antibody (CL488-27260) at dilution of 1:800.

• 

  IF Staining of Caco-2 using CL488-27260

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using CoraLite® Plus 488 Occludin antibody (CL488-27260) at dilution of 1:1000.

• 

  FC experiment of MCF-7 using CL488-27260

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Occludin (CL488-27260) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen, Caco-2-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 3 publications below

Produktinformation

CL488-27260 bindet in IF/ICC, FC (Intra) Occludin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Occludin fusion protein Ag26173
• Vollständiger Name: occludin
• Berechnetes Molekulargewicht: 522 aa, 59 kDa
• GenBank-Zugangsnummer: BC029886
• Gene symbol: Occludin
• Gene ID (NCBI): 4950
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Publikationen

Species	Application	Title
human	IF	J Neurodev Disord; Rescue of impaired blood-brain barrier in tuberous sclerosis complex patient derived neurovascular unit; Authors - Jacquelyn A Brown
mouse	IF	Cell; The choroid plexus synergizes with immune cells during neuroinflammation; Authors - Huixin Xu
	IF	Int J Pharm; A novel pH-responsive berberine-loaded attapulgite microsphere for IBD therapy via the regulation of gut immunity and flora; Authors - Shiwen Gong