Osteoglycin Polyklonaler Antikörper

Osteoglycin Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12755-1-PBS

Synonyme

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  WB analysis of NIH/3T3 using 12755-1-AP (same clone as 12755-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 12755-1-AP (same clone as 12755-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12755-1-AP (same clone as 12755-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 12755-1-AP (same clone as 12755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 12755-1-AP (Osteoglycin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 12755-1-AP (same clone as 12755-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 12755-1-AP (Osteoglycin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12755-1-AP (same clone as 12755-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Osteoglycin antibody (12755-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12755-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Osteoglycin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Osteoglycin fusion protein Ag3484
• Vollständiger Name: osteoglycin
• Berechnetes Molekulargewicht: 298 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34-40 kDa
• GenBank-Zugangsnummer: BC037273
• Gene symbol: OGN
• Gene ID (NCBI): 4969
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.