Osteopontin Polyklonaler Antikörper

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• KD/KO Validated

Osteopontin Polyklonal Antikörper für WB, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 30200-1-AP

Synonyme

OPN, 2AR, Bone sialoprotein 1, BSPI, Eta-1

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  WB analysis using 30200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30200-1-AP (Osteopontin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 30200-1-AP

  WB result of Osteopontin antibody (30200-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Osteopontin transfected RAW 264.7 cells.

• 

  WB analysis using 30200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30200-1-AP (Osteopontin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30200-1-AP (Osteopontin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse stomach using 30200-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using Osteopontin antibody (30200-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 30200-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Osteopontin antibody (30200-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of RAW 264.7 using 30200-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using Osteopontin antibody (30200-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, humanes Nierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IF-P: Maus-Magengewebe
• Erfolgreiche Detektion in IF/ICC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

30200-1-AP bindet in WB, IHC, IF/ICC, IF-P, ELISA Osteopontin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Osteopontin fusion protein Eg0317
• Vollständiger Name: secreted phosphoprotein 1
• Berechnetes Molekulargewicht: 32kd
• Beobachtetes Molekulargewicht: 32 kDa, 60-70 kDa
• GenBank-Zugangsnummer: NM_001204233.1
• Gene symbol: Osteopontin
• Gene ID (NCBI): 20750
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Osteopontin (OPN) is also named as 2AR, minopontin, bone sialoprotein 1, Spp1, Eta-1 and Op, and belongs to the osteopontin family. Osteopontin is major non-collagenous bone protein that binds tightly to hydroxyapatite. It is a highly phosphorylated glycophosphoprotein having acidic characteristics and rich in aspartic acid (PMID: 30003880). And it acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (PMID:2351930, PMID:10657301). OPN, a multifunctional protein, has important functions on cardiovascular diseases, cancer, diabetes and kidney stone diseases and in the process of inflammation, biomineralization, cell viability and wound healing (PMID: 30003880). Osteopontin (OPN) is a pleiotropic glycoprotein expressed in various cell types in animals and in humans, including bone, immune, smooth muscle, epithelial and endothelial cells. Moreover, OPN is found in kidneys (in the thick ascending limbs of the loop of Henle and in distal nephrons) and urine (PMID:30456594). Osteopontin (OPN) is an acidic 60-70 kDa glycoprotein that is cleaved by proteases to yield 45-kDa and 24-kDa fragments (PMID: 10491621, PMID: 1995615)

Publikationen

Species	Application	Title
rat	WB	Int J Biol Macromol; Cranial repair-promoting effect of oxidised sodium alginate/amino gelatine injectable hydrogel loaded with deer antler blood peptides; Authors - Taojing Yu
human	WB	Biomedicines; Mechanistic Insights into Salvigenin for Glucocorticoid-Induced Femoral Head Osteonecrosis: A Network Pharmacology and Experimental Study; Authors - Zhengjie Zhu
rat,mouse	WB	J Transl Med; The surface protein Gbp of Fusobacterium nucleatum inhibits osteogenic differentiation by inactivating the Wnt/β-catenin pathway via binding to Annexin A2; Authors - Rui Dong
human	WB	J Gastrointest Oncol; SPP1 promotes tumor progression in esophageal carcinoma by activating focal adhesion pathway; Authors - Jianjun Sun
rat	WB	J Orthop Surg Res; Uniaxial static strain enhances osteogenic and angiogenic potential under hypoxic conditions in distraction osteogenesis; Authors - Lifang Zhang
mouse	IHC	Osteoarthritis Cartilage; M2 macrophages regulate nucleus pulposus cell extracellular matrix synthesis through the OPN-CD44 axis in intervertebral disc degeneration; Authors - Zhiwen Tao