Osteopontin Rekombinanter Antikörper

Osteopontin Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240250F11

Kat-Nr. : 83413-4-PBS

Synonyme

Spp1, Opn, 240250F11

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  Sandwich ELISA standard curve of MP00400-2

  Sandwich ELISA standard curve of MP00400-2, mouse/rat Osteopontin Recombinant Matched Antibody Pair - PBS only. 83413-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0317. 83413-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

• 

  Sample test of MP00400-2

  Serum of sixteen mice was measured. The Osteopontin concentration of detected samples was determined to be 32.0 ng/mL with a range of 6.2 - 53.0 ng/mL.

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  Sample test of MP00400-2

  Serum of seven rats was measured. The Osteopontin concentration of detected samples was determined to be 12.8 ng/mL with a range of 1.1 - 43.0 ng/mL.

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  Sample test of MP00400-2

  J774A.1 mouse reticulum cell sarcoma macrophage cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for mouse/rat Osteopontin and measured 20.0 ng/mL.

• 

  Cytometric bead array standard curve of MP00400-2

  Cytometric bead array standard curve of MP00400-2, MOUSE Osteopontin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83413-5-PBS. Detection antibody: 83413-4-PBS. Standard: Eg0317. Range: 0.313-40 ng/mL.

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Geprüfte Anwendungen

Produktinformation

83413-4-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Osteopontin und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: secreted phosphoprotein 1
• Berechnetes Molekulargewicht: 32kd
• GenBank-Zugangsnummer: NM_001204233.1
• Gene symbol: Osteopontin
• Gene ID (NCBI): 20750
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.