Osteopontin Rekombinanter Antikörper

Osteopontin Rekombinant Antikörper für IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240250H3

Kat-Nr. : 83413-5-PBS

Synonyme

Spp1, 240250H3, Opn

---

• 

  Sandwich ELISA standard curve of MP00400-2

  Sandwich ELISA standard curve of MP00400-2, mouse/rat Osteopontin Recombinant Matched Antibody Pair - PBS only. 83413-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0317. 83413-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

• 

  Sample test of MP00400-2

  Serum of sixteen mice was measured. The Osteopontin concentration of detected samples was determined to be 32.0 ng/mL with a range of 6.2 - 53.0 ng/mL.

• 

  Sample test of MP00400-2

  Serum of seven rats was measured. The Osteopontin concentration of detected samples was determined to be 12.8 ng/mL with a range of 1.1 - 43.0 ng/mL.

• 

  Sample test of MP00400-2

  J774A.1 mouse reticulum cell sarcoma macrophage cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for mouse/rat Osteopontin and measured 20.0 ng/mL.

• 

  IF Staining of RAW 264.7 using 83413-5-RR (same clone as 83413-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Osteopontin antibody (83413-5-RR, Clone: 240250H3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83413-5-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00400-2

  Cytometric bead array standard curve of MP00400-2, MOUSE Osteopontin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83413-5-PBS. Detection antibody: 83413-4-PBS. Standard: Eg0317. Range: 0.313-40 ng/mL.

• 

  Affinity and Kinetic Characterization of 83413-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83413-5-RR against Mouse Osteopontin were performed. The affinity constant is 4.36 nM.

---

Geprüfte Anwendungen

Produktinformation

83413-5-PBS bindet in IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Osteopontin und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Osteopontin fusion protein Eg0317
• Vollständiger Name: secreted phosphoprotein 1
• Berechnetes Molekulargewicht: 32kd
• GenBank-Zugangsnummer: NM_001204233.1
• Gene symbol: Osteopontin
• Gene ID (NCBI): 20750
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Osteopontin (OPN) is also named as 2AR, minopontin, bone sialoprotein 1, Spp1, Eta-1 and Op, and belongs to the osteopontin family. Osteopontin is major non-collagenous bone protein that binds tightly to hydroxyapatite. It is a highly phosphorylated glycophosphoprotein having acidic characteristics and rich in aspartic acid (PMID: 30003880). And it acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (PMID:2351930, PMID:10657301). OPN, a multifunctional protein, has important functions on cardiovascular diseases, cancer, diabetes and kidney stone diseases and in the process of inflammation, biomineralization, cell viability and wound healing (PMID: 30003880). Osteopontin (OPN) is a pleiotropic glycoprotein expressed in various cell types in animals and in humans, including bone, immune, smooth muscle, epithelial and endothelial cells. Moreover, OPN is found in kidneys (in the thick ascending limbs of the loop of Henle and in distal nephrons) and urine (PMID:30456594). Osteopontin (OPN) is an acidic 60-70 kDa glycoprotein that is cleaved by proteases to yield 45-kDa and 24-kDa fragments (PMID: 10491621, PMID: 1995615)