Osteopontin Rekombinanter Antikörper

Osteopontin Rekombinant Antikörper für IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

240250A1

Kat-Nr. : 83413-6-RR

Synonyme

Spp1, 240250A1, Opn

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  IF Staining of mouse stomach using 83413-6-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using Osteopontin antibody (83413-6-RR, Clone: 240250A1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 83413-6-RR

  Immunofluorescent analysis of (4% PFA) fixed LPS and Brefeldin A treated RAW 264.7 cells using Osteopontin antibody (83413-6-RR, Clone: 240250A1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83413-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83413-6-RR against Mouse Osteopontin were performed. The affinity constant is 2.19 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maus-Magengewebe
• Erfolgreiche Detektion in IF/ICC: mit LPS und Brefeldin A behandelte RAW 264.7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83413-6-RR bindet in IF/ICC, IF-P, ELISA Osteopontin und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Osteopontin fusion protein Eg0317
• Vollständiger Name: secreted phosphoprotein 1
• Berechnetes Molekulargewicht: 32kd
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: NM_001204233.1
• Gene symbol: Osteopontin
• Gene ID (NCBI): 20750
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Osteopontin (OPN) is also named as 2AR, minopontin, bone sialoprotein 1, Spp1, Eta-1 and Op, and belongs to the osteopontin family. Osteopontin is major non-collagenous bone protein that binds tightly to hydroxyapatite. It is a highly phosphorylated glycophosphoprotein having acidic characteristics and rich in aspartic acid (PMID: 30003880). And it acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (PMID:2351930, PMID:10657301). OPN, a multifunctional protein, has important functions on cardiovascular diseases, cancer, diabetes and kidney stone diseases and in the process of inflammation, biomineralization, cell viability and wound healing (PMID: 30003880). Osteopontin (OPN) is a pleiotropic glycoprotein expressed in various cell types in animals and in humans, including bone, immune, smooth muscle, epithelial and endothelial cells. Moreover, OPN is found in kidneys (in the thick ascending limbs of the loop of Henle and in distal nephrons) and urine (PMID:30456594).