P-cadherin Rekombinanter Antikörper

P-cadherin Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251775F5

Kat-Nr. : 86776-1-RR

Synonyme

Cadherin 3, Cadherin-3, CDH3, CDHP, HJMD

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  WB analysis using 86776-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86776-1-RR (CDH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86776-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86776-1-RR (CDH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 86776-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using P-cadherin antibody (86776-1-RR, Clone: 251775F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HaCaT-Zellen, HCT 116-Zellen, Mausembryogewebe
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86776-1-RR bindet in WB, IF/ICC, ELISA P-cadherin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: P-cadherin fusion protein Eg5236
• Vollständiger Name: cadherin 3, type 1, P-cadherin (placental)
• Berechnetes Molekulargewicht: 91 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: NM_001793.5
• Gene symbol: P-cadherin
• Gene ID (NCBI): 1001
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.