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P16-INK4A Polyklonaler Antikörper
P16-INK4A Polyklonal Antikörper für FC, IHC, IP, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (2)
Anwendung
WB, IP, IHC, FC, Cell treatment, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10883-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, PC-3-Zellen |
| Erfolgreiche IP | HEK-293-Zellen |
| Erfolgreiche Detektion in IHC | humanes Zervixkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in FC | HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:1000-1:4000 |
| Durchflusszytometrie (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 246 publications below |
| IP | See 1 publications below |
| FC | See 1 publications below |
Produktinformation
10883-1-AP bindet in WB, IP, IHC, FC, Cell treatment, ELISA P16-INK4A und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human, Affe, Hund |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | P16-INK4A fusion protein Ag1328 |
| Vollständiger Name | cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) |
| Berechnetes Molekulargewicht | 16 kDa |
| Beobachtetes Molekulargewicht | 16-18 kDa |
| GenBank-Zugangsnummer | BC021998 |
| Gene symbol | CDKN2A |
| Gene ID (NCBI) | 1029 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Background
p16 is an important cell cycle regulator and acts as a tumor suppressor. It may also be referred to as one of a number of synonyms, including p16INK4a and cyclin-dependent kinase inhibitor 2A.
What is the molecular weight of P16?
16kDa. P16 is encoded by the CDKN2A gene in humans and is a chain comprising 148 amino acids.
What is the function of p16?
P16 inhibits cells from progressing from G1 into S phase, binding to cyclin-dependent kinase 4 (CDK4) and inhibiting its kinase ability, so that it cannot phosphorylate the retinoblastoma tumor suppressor (RB). Without this phosphorylation, RB does not activate downstream genes, so the G1/S checkpoint cannot be passed and the cell does not proliferate (PMID: 8259215).
What is the role of p16 in senescence?
In senescence, cells are irreversibly arrested in the cell cycle. P16 is expressed more highly in aging tissue, is associated with intrinsic cellular aging signals such as telomere shortening, and can therefore be used as a marker of senescence (PMID: 9244355; PMID: 19535234). Due to its role in cell cycle arrest, p16 drives the initiation and maintenance of a cellular senescent phenotype.
What is the role of p16 in cancer?
As a negative regulator of proliferation, p16 is a known tumor suppressor. Mutations in the CDKN2A gene that lead to inactivation of p16 protein have been associated with an increased risk of cancer and are often observed in primary tumors and in cancer cell lines (PMID: 9508208). The inactivation of p16 has been shown to be a key early stage of tumor progression. In a small number of tumor types that are caused by the human papilloma virus (HPV), p16 is in fact overexpressed when RB is inactivated, releasing p16 and causing an accumulation (PMID: 21297668).
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for P16-INK4A antibody 10883-1-AP | Protokoll herunterladen |
| IHC protocol for P16-INK4A antibody 10883-1-AP | Protokoll herunterladen |
| IP protocol for P16-INK4A antibody 10883-1-AP | Protokoll herunterladen |
| FC protocol for P16-INK4A antibody 10883-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Science PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation. | ||
Nat Genet Early TP53 alterations engage environmental exposures to promote gastric premalignancy in an integrative mouse model. | ||
Cancer Cell mTORC1 Activation Blocks Braf(V600E)-Induced Growth Arrest but Is Insufficient for Melanoma Formation. | ||
Circulation Vascular Smooth Muscle Cell Senescence Promotes Atherosclerosis and Features of Plaque Vulnerability. | ||
Cell Metab SREBP1c-PARP1 axis tunes anti-senescence activity of adipocytes and ameliorates metabolic imbalance in obesity. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Susanne (Verified Customer) (10-11-2022) | blocking: ROTIblock Incubation over night 4°C
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FH Malak (Verified Customer) (04-22-2021) | Unfortunately, with this antibody, I didn't get a bad at 16 KD, however it was higher (around 20 KD). The suppliers were so professional and offered their help to optimize this antibody.
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FH Joshua (Verified Customer) (03-12-2020) | MDCKs fixed in 4% paraformaldehyde and stained overnight at 4 C. Excellent, bright stain.
 |
FH Kyle (Verified Customer) (02-28-2019) | Clean bands, low background
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