CDKN2A/P16-INK4A Monoklonaler Antikörper
CDKN2A/P16-INK4A Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human und mehr (1)
Anwendung
WB, IHC, IF/ICC, ELISA
Konjugation
Unkonjugiert
CloneNo.
1C9D3
Kat-Nr. : 60626-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | SiHa cells, HEK-293-Zellen, HeLa-Zellen, TF-1-Zellen |
| Erfolgreiche Detektion in IHC | humanes Tonsillitisgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunhistochemie (IHC) | IHC : 1:2500-1:10000 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 2 publications below |
Produktinformation
60626-1-Ig bindet in WB, IHC, IF/ICC, ELISA CDKN2A/P16-INK4A und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human, Maus |
| Wirt / Isotyp | Maus / IgG2a |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | CDKN2A/P16-INK4A fusion protein Ag1328 |
| Vollständiger Name | cyclin-dependent kinase inhibitor 2A |
| Berechnetes Molekulargewicht | 16 kDa |
| Beobachtetes Molekulargewicht | 16 kDa |
| GenBank-Zugangsnummer | BC021998 |
| Gene symbol | CDKN2A |
| Gene ID (NCBI) | 1029 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
P16-INK4A is also named as CDKN2A, MLM, Tumor suppressor ARF, Alternative reading frame. The tumor suppressor protein p16Ink4a (encoded from the CDKN2A locus) is often transcriptionally activated in cells undergoing senescence and is one of the main regulators of this program, and it is upregulated in multiple tissues during aging (PMID:17055429). p16-Ink4a is the principal member of the Ink4 family of CDK inhibitors. p16-Ink4a contributes to the regulation of cell cycle progression by inhibiting the S phase. p16Ink4a binds to CDK4/6, inhibiting cyclin D-CDK4/6 complex formation and CDK4/6-mediated phosphorylation of Rb family members. Expression of p16-Ink4a maintains the Rb family members in a hypophosphorylated state, which promotes binding to E2F1 and leads to G1 cell cycle arrest (PMID: 21297668).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for CDKN2A/P16-INK4A antibody 60626-1-Ig | Protokoll herunterladen |
| IHC protocol for CDKN2A/P16-INK4A antibody 60626-1-Ig | Protokoll herunterladenl |
| IF protocol for CDKN2A/P16-INK4A antibody 60626-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Nat Aging Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer | ||
Hum Vaccin Immunother Nanovaccine loaded with seno-antigen target senescent cells to improve metabolic disorders of adipose tissue and cardiac dysfunction |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Marisa (Verified Customer) (07-31-2025) | We used the p16 antibody from Proteintech for immunofluorescence on mouse brain, followed by confocal microscopy. The antibody produced a strong, specific signal and Minimal background and high signal-to-noise ratio made it easy to interpret.
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