CDKN2A/P16-INK4A Rekombinanter Antikörper

CDKN2A/P16-INK4A Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241438C7

Kat-Nr. : 81373-8-PBS

Synonyme

p16, p16 INK4, P16 INK4A, p16-INK4, p16INK4A

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  WB analysis using 81373-8-RR (same clone as 81373-8-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81373-8-RR (P16-INK4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81373-8-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 81373-8-RR (same clone as 81373-8-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 81373-8-RR (P16-INK4A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81373-8-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 81373-8-RR (same clone as 81373-8-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81373-8-RR (P16-INK4A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81373-8-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 81373-8-RR (same clone as 81373-8-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDKN2A/P16-INK4A antibody (81373-8-RR, Clone: 241438C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81373-8-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81373-8-RR

  Biolayer interferometry (BLl) kinetic assays of 81373-8-RR against Human P16-INK4A were performed. The affinity constant is 0.336 nM.

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Geprüfte Anwendungen

Produktinformation

81373-8-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CDKN2A/P16-INK4A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CDKN2A/P16-INK4A fusion protein Ag1328
• Vollständiger Name: cyclin-dependent kinase inhibitor 2A
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC021998
• Gene symbol: CDKN2A
• Gene ID (NCBI): 1029
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDKN2A generates several transcript variants which differ in their first exons. At least three alternatively-spliced variants encoding distinct proteins proteins were reported. Two of them named p16-INK4 and p14 are sharing 50% identity. P16 plays an essential role in regulating the cell cycle, and mutations in p16 increase the risk of developing various cancers, including melanoma. Long-term p16(INK4a) expression pushes cells to enter senescence, an irreversible cell-cycle arrest that precludes the growth of would-be cancer cells but also contributes to cellular aging (PMID: 24136988).