CDKN2A/P16-INK4A Rekombinanter Antikörper

CDKN2A/P16-INK4A Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241438C7

Rezensionen (1)

Kat-Nr. : 81373-8-RR

Synonyme

p16, p16 INK4, P16 INK4A, p16-INK4, p16INK4A

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  WB analysis using 81373-8-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81373-8-RR (P16-INK4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 81373-8-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81373-8-RR (P16-INK4A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 81373-8-RR

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 81373-8-RR (P16-INK4A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 81373-8-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDKN2A/P16-INK4A antibody (81373-8-RR, Clone: 241438C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 81373-8-RR

  Biolayer interferometry (BLl) kinetic assays of 81373-8-RR against Human P16-INK4A were performed. The affinity constant is 0.336 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, TF-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81373-8-RR bindet in WB, IHC, IF/ICC, ELISA CDKN2A/P16-INK4A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CDKN2A/P16-INK4A fusion protein Ag1328
• Vollständiger Name: cyclin-dependent kinase inhibitor 2A
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC021998
• Gene symbol: CDKN2A
• Gene ID (NCBI): 1029
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDKN2A generates several transcript variants which differ in their first exons. At least three alternatively-spliced variants encoding distinct proteins proteins were reported. Two of them named p16-INK4 and p14 are sharing 50% identity. P16 plays an essential role in regulating the cell cycle, and mutations in p16 increase the risk of developing various cancers, including melanoma. Long-term p16(INK4a) expression pushes cells to enter senescence, an irreversible cell-cycle arrest that precludes the growth of would-be cancer cells but also contributes to cellular aging (PMID: 24136988).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jordi (Verified Customer) (08-01-2025)

Antibody works very well in WB detection p16 band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Breast cancer cell lines (T47D and MCF7) and Colon cancer cell lines (SW480 and SW620)