P21 Polyklonaler Antikörper

P21 Polyklonal Antikörper für ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 10240-1-AP

Synonyme

CDKN1A, P21;CDKN1A, CDK-interacting protein 1, MDA-6, Cyclin-dependent kinase inhibitor 1

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Geprüfte Anwendungen

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

10240-1-AP bindet in WB, ELISA P21 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P21 fusion protein Ag0308
• Vollständiger Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Berechnetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC000312
• Gene symbol: p21
• Gene ID (NCBI): 1026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

P21 (also known as CIP1, WAF1) is a cyclin-dependent kinase inhibitor. P21 binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of P21 is induced by wild-type but not mutant p53 protein, through which P21 mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. P21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

Publikationen

Species	Application	Title
human	WB	PLoS One; FLASH knockdown sensitizes cells to Fas-mediated apoptosis via down-regulation of the anti-apoptotic proteins, MCL-1 and Cflip short.; Authors - Chen Song S
human,mouse	WB	Head Neck; TMEM252 inhibits epithelial-mesenchymal transition and progression in papillary thyroid carcinoma by regulating Notch1 expression; Authors - Shuyong Zhang

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