P21 Polyklonaler Antikörper

P21 Polyklonal Antikörper für IF,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 10240-1-AP

Synonyme

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Galerie der Validierungsdaten

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  IHC staining of human breast cancer using 10240-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10240-1-AP (P21 antibody) at dilution of 1:50 (under 25x lens).

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  IF Staining of myotome of a somite at E11.5 using 10240-1-AP

  IF result of anti-P21(10240-1-AP) in the myotome of a somite at E11.5 by Dr. Zalc A. and Relaix F. (UPMC - UMRS 787).

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  IF Staining of SH-SY5Y using 10240-1-AP

  Immunofluorescent analysis of SH-SY5Y cells, using CDKN1A antibody 10240-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: myotome of a somite at E11.5, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

10240-1-AP bindet in WB, IF,ELISA P21 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P21 fusion protein Ag0308
• Vollständiger Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Berechnetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC000312
• Gene symbol: CDKN1A
• Gene ID (NCBI): 1026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.

Publikationen

Species	Application	Title
human	WB	PLoS One; FLASH knockdown sensitizes cells to Fas-mediated apoptosis via down-regulation of the anti-apoptotic proteins, MCL-1 and Cflip short.; Authors - Chen Song S