P21 Rekombinanter Antikörper

P21 Rekombinant Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3N14

Kat-Nr. : 82669-2-PBS

Synonyme

CDKN1A, P21;CDKN1A, 3N14, CDK-interacting protein 1, CIP1

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  WB analysis using 82669-2-RR (same clone as 82669-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82669-2-RR (P21 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82669-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 82669-2-RR (same clone as 82669-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82669-2-RR (P21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82669-2-PBS in a different storage buffer formulation.

• 

  IP experiment of MCF-7 using 82669-2-RR (same clone as 82669-2-PBS)

  IP result of anti-P21 (IP:82669-2-RR, 4ug; Detection:82669-2-RR 1:800) with MCF-7 cells lysate 760 ug. This data was developed using the same antibody clone with 82669-2-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 82669-2-RR (same clone as 82669-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using P21 antibody (82669-2-RR, Clone: 3N14 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82669-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82669-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82669-2-RR against Human P21 were performed. The affinity constant is 112 nM.

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Geprüfte Anwendungen

Produktinformation

82669-2-PBS bindet in WB, IF/ICC, IP, Indirect ELISA P21 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: P21 fusion protein Ag0368
• Vollständiger Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC000275
• Gene symbol: p21
• Gene ID (NCBI): 1026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.