P21 Rekombinanter Antikörper

P21 Rekombinant Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

3N14

Kat-Nr. : 82669-2-RR

Synonyme

CDKN1A, P21;CDKN1A, 3N14, CDK-interacting protein 1, CIP1

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  WB analysis using 82669-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82669-2-RR (P21 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 82669-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82669-2-RR (P21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 82669-2-RR

  IP result of anti-P21 (IP:82669-2-RR, 4ug; Detection:82669-2-RR 1:800) with MCF-7 cells lysate 760 ug.

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  IF Staining of MCF-7 using 82669-2-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using P21 antibody (82669-2-RR, Clone: 3N14 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 82669-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82669-2-RR against Human P21 were performed. The affinity constant is 112 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HCT 116-Zellen, HeLa-Zellen, HepG2-Zellen, HUVEC-Zellen, MCF-7-Zellen, THP-1-Zellen, U-87 MG-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

82669-2-RR bindet in WB, IF/ICC, IP, ELISA P21 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: P21 fusion protein Ag0368
• Vollständiger Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC000275
• Gene symbol: p21
• Gene ID (NCBI): 1026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

Publikationen

Species	Application	Title
	WB	Nat Commun; Foxk1 and Foxk2 promote cardiomyocyte proliferation and heart regeneration; Authors - Dongcheng Cai
human	WB	J Biol Chem; The P53-P21-RB1 pathway promotes BRD4 degradation in liver cancer through USP1; Authors - Neng Li